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Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe BABT
Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious bud; Anatomy; Morphogenesis; Zingiberaceae.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014
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In vitro organogenesis in watermelon cotyledons PAB
Krug,Maria Graziela Zagatto; Stipp,Liliane Cristina Libório; Rodriguez,Adriana Pinheiro Martinelli; Mendes,Beatriz Madalena Januzzi.
The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-1) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrullus lanatus; Histological study; Adventitious bud.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900004
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In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia Scientia Agricola
Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli.
Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious bud; Rooting; Genetic transformation.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004
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